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KMID : 1101220030350010014
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2003 Volume.35 No. 1 p.14 ~ p.21
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Characteristics on the Toxicity of Sonicated Bacterial Extracts, Lipopolysaccharides, and Bacterial Growth Supernatants of Prevotella nigrescens
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Kim Eun-Sook
Kim Shin-Moo
Cho Ji-Hyun
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Abstract
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The purpose of this study was to investigate the toxicities and toxic activities of bacterial preparations from Prevotella nigrescens. Supernatants, sonicated bacterial extracts, and lipopolysaccharides(LPS) were used to compare the possible specific sensitivities of human red blood cells(RBCs) toxicity against P. nigrescens ATCC 33592 isolates. The clinical isolates of P. nigrescens were cultured from infected root canals and identified using polymerase chain reactions of 16S rRNA primers and an ATCC type strain(33592). The toxicity of bacterial growth medium supernatants, sonicated bacterial extracts, and LPSs on RBCs were expressed as a percentage of the positive control(distilled water plus erythrocytes), which was considered to be 100% lysis. The toxicity of RBCs in bacterial growth supernatant detected P. nigrescens ATCC 33592 at 30.5% and P. nigrescens clinical isolate at 27.1%. Sonicated bacterial extracts had concentrated toxicity at dosages of 30㎍/ml, 50㎍/ml, and 100㎍/ml. The LPS fractions had no toxicity. These results suggest that the characteristics of the toxicity of RBCs against P. nigrescens causes lysis of erythrocytes in sonicated bacterial extract.
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KEYWORD
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Prevotella nigrescens, PCR, Toxicity, LPS
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